Andrew H.

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Endogenous Dark Chromophore Imaging via Modulated Stimulated Emission

Andrew H. posted an article on - Nov 11, 2009, 10:21 pm
Here is an interesting paper, Imaging chromophores with undetectable fluorescence by stimulated emission microscopy, from Sunny Xie’s group.  They pump the sample with a excitation laser while...
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Three Cheers for GCaMP : Optogenetic Brain Reading

Andrew H. posted an article on - Nov 9, 2009, 5:54 pm
Three papers are out online in Nature Methods that show big improvements in calcium imaging with genetically encoded sensors.  They are are based on the fluorescence intensity indicator, GCaMP.   GC...
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Playing Quake with a Real Mouse

Andrew H. posted an article on - Oct 14, 2009, 1:21 pm
Most people play Quake with a computer mouse, but researchers in David Tank’s lab at Princeton have done it with a living mouse, AND they are recording the intracellular activity of individual n...
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Automated ROI analysis for calcium imaging

Andrew H. posted an article on - Oct 2, 2009, 6:04 pm
One of the most time consuming and frustrating tasks associated with fluorescence imaging in the brain is picking out your regions of interest.  Which pixels do you include in as part of the cell and...
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Optogenetic induction of memory recall

Andrew H. posted an article on - Sep 18, 2009, 2:40 pm
Speaking of reactivating specific memories, at the 2009 Society for Neuroscience meeting, Matteo Rizzi of Michael Häusser’s lab is presenting the realization of an idea that has been floating a...
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Light-switchable protein interactions

Andrew H. posted an article on - Sep 16, 2009, 11:06 am
A fully genetically-encoded approach to light-activated transcription is getting closer now that a new, generalizable method of light-switchable protein interactions has been published.  In Nature&...
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Voltage imaging with sub-millisecond, single-action potential resolution

Andrew H. posted an article on - Jul 27, 2009, 10:28 am
I have been itching to post about this work since David DiGregorio presented it at a meeting at Janelia last year. His group’s results, Submillisecond Optical Reporting of Membrane Potentials In...
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Annual Reviews worth reading

Andrew H. posted an article on - Jul 22, 2009, 2:11 pm
Annual Reviews of Neuroscience published their 2009 issue recently.  These articles are usually a great way to catch up with a field, particularly when they are recently published.  Here are a few t...
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Neurons controlled by DREADD

Andrew H. posted an article on - Jul 16, 2009, 9:17 am
A big advance in non-invasive neuronal remote control was published today in Neuron. Several groups have been working on expressing non-endogenous or customized receptors into neurons so that specific...
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Philosophy : Can a Biologist Reverse-Engineer a Radio?

Andrew H. posted an article on - Jul 15, 2009, 10:14 am
Every week or two one of the post-doc’s in our lab passes around a paper that is as much philosophy of science as experimental result.  One that recently made the rounds is Can a Biologist Fix ...
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High-resolution deep-brain two-photon imaging

Andrew H. posted an article on - Jul 14, 2009, 11:48 pm
Mark Schnitzer, who recently became an HHMI Investigtor, has a new paper out on improved optics for his group’s miniature probe microscopes.  Mark has been pioneering these tiny probes to do op...
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Photoactivated Transcription Revisted

Andrew H. posted an article on - Jul 14, 2009, 10:46 pm
Looks like there has been some new results in the field of photoactivated transcription.  Unlike the fully genetically-encoded systems reviewed in a Journal Club, this uses a hybrid genetic and small...
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